Urine colony counts inoculate the agar plate to perform a urine colony count inoculate the agar plates with one micro liter of urine. The sterile loop is calibrated to hold 1 micro liter past this loop down the center of the plate and carefully spread the urine over the entire plate incubate, the plates overnight at 37 degrees. Celsius let's, take a look on account setup requires the agar plate to grow the organisms on a 1 micro liter loop and the urine specimen you take the 1 micro liter.

Calibrated loop, sis, sterile open carefully and will pick up one loop full, which is calibrated to be 1 micro liter of specimen. It is streak straight down the center of the plate and then back-and-forth to spread out the colony. So that you can get a colony count reading after it has incubated diagram of urine culture, inoculation procedure. First streak down the middle of the plate. Next streak from side to side to distribute the organisms evenly on the agar plate reading the plates after the plates. Have incubated count each colony on the plate, each colony represents one bacterium, which forms a colony and is called a colony forming unit counting the colonies count.

The number of colonies on the plate. This is the number of colonies per 1 micro. Liter of urine use the conversion factor to convert 1 micro liter to one milliliter, counting the colonies.

The calculation colonies per plate 54, amount, plated 1 micro, liter, here's, the calculation, the micro liters cancel. And you are left with 54,000. Colonies per 1 milliliter note, if you counted 50 or 52 or 56 for this sample, no problem just use the number you counted in your calculations, your answer will be different from the answer above. But the exponent will be the same also always remember to put the units of colonies and milliliters in your calculations.

And in your answer, counting the colonies. The number of colonies on this plate is greater than 100. The number used for your calculations is greater than 100 colonies per one microliter. Use your conversion factor to convert this value to colonies per milliliter of urine, counting the colonies, the calculation colonies per plate greater than 100 amounts plated, one microliter, here's the calculation, the micro liters cancel.

And you are left with greater than 100,000 colonies per one milliliter note, remember to put the units of colonies and milliliters in your calculations. And in your answer, you must use the greater than sign in your answer count. The colonies count, the number of.

Colonies on this plate. This is the number of colonies per 1 micro. Liter of urine use the conversion factor to convert 1 micro liter to one milliliter, counting the colonies' calculation colonies per plate, 75 amount, plated 1 micro liter. Here is the calculation. The micro liters cancel, and you're left with 75,000 colonies per one milliliter note. If you count in a slightly different number, no problem just use the number you counted in your calculations, your answer will be different from the answer. Above, but the exponent will be the same also always remember to put the units of colonies and milliliters in your calculations, and in your answer, counting the colonies.

Now count the number of colonies on this plate. This is the number of colonies per 1 micro, liter of urine use the conversion factor to convert 1 micro liter to one milliliter, counting the colonies' calculation colonies per plate greater than 100, a much plated. One microliter, here's, the calculation, the microliters cancel, and you're. Left with greater than 100,000 colonies per one milliliter. In summary, certain criteria are used to evaluate a clean catch midstream, urine colony counts to determine if it represents an infection.

A few bacteria will typically be present on the agar from a clean catch urine colony count because the urine is contaminated with skin organisms as it leaves the urethra. If there is an infection, it is usually due to just one type of organism cultures with more than three organisms present on the agar are. Most likely contaminated the following results are typical of a urinary tract infection high colony counts of one organism, especially greater than 100,000 are indicative of an infection.

Lower counts can also indicate an infection in some circumstances. White blood cells are seen on the microscopic evaluation of the urine very few epithelial cells are seen on the microscopic evaluation. All urine cultures should be taken to the laboratory or stored in the refrigerator as soon as possible after. Collection to replication of organisms in the sample. This concludes this activity, urine colony counts. You.